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Latka A 1 ,. Search articles by 'Barbara Maciejewska'.

Maciejewska B 1 ,. Vandenheuvel D 2 ,.

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Olszak T 1 ,. Search articles by 'Yves Briers'. Briers Y 3 ,. Search articles by 'Giles S Holt'. Holt GS 4 ,. Valvano MA 5 ,.


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Search articles by 'Rob Lavigne'. Lavigne R 2 ,. Smith DL 4 ,. Drulis-Kawa Z 1. Affiliations 5 authors 1. Share this article Share with email Share with twitter Share with linkedin Share with facebook. Appl Microbiol Biotechnol.

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Published online Oct PMID: Holt , 4 Miguel A. Valvano , 5 Rob Lavigne , 2 Darren L. Smith , 4 and Zuzanna Drulis-Kawa 1. Giles S. Miguel A. Darren L. Author information Article notes Copyright and License information Disclaimer.

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Electronic supplementary material The online version of this article doi Phage isolation and propagation Phage AP3 was isolated from a natural wastewater treatment plant irrigated fields in Wroclaw Poland as previously described Olszak et al. Isolate no. Open in a separate window. Burst size experiments and sensitivity of phage particles to temperature, chloroform, and pH A one-step growth curve of the isolated Burkholderia phage was performed as described Pajunen et al. The emergence of phage-resistant clones The B. Phage isolation and morphology Phage AP3 was isolated in a water sample from irrigated fields and propagated on the clinical B.

Galleria infection treatment assay and the emergence of phage-resistant mutants The wax moth larvae model was used to evaluate in vivo the antibacterial efficacy of AP3 temperate phage in the eradication of B. Table 2 B. ESM 1 1. Abedon ST. Bacteriophages and biofilms: ecology, phage therapy, plaques. New York: Nova Science Publishers; Classification of bacteriophages. In: Calendar R, editor. The bacteriophages. New York: Oxford University Press; New York: Interscience; Basic local alignment search tool. J Mol Biol. Nucl Acids Res. Spanin function requires subunit homodimerization through intermolecular disulfide bonds.

Mol Microbiol. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Burkholderia cenocepacia induces neutrophil necrosis in chronic granulomatous disease. J Immunol. J Bacteriol. Efficacy of bacteriophage therapy in a model of Burkholderia cenocepacia pulmonary infection. J Infect Dis. Exploiting molecular virulence determinants in Burkholderia to develop vaccine antigens. Curr Med Chem. Spe I restriction enzyme displays greater discriminatory power than Xba I enzyme does in a pulsed-field gel electrophoresis study on clinical Burkholderia pseudomallei isolates.

Jpn J Infect Dis. Antibacterial efficacy of temperate phage-mediated inhibition of bacterial group motilities.

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Antimicrob Agents Chemother. Phenotypic characterization of an international Pseudomonas aeruginosa reference panel: strains of cystic fibrosis CF origin show less in vivo virulence than non-CF strains. A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections. Sci Rep. Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence. Clin Microbiol Infect. Learning from bacteriophages—advantages and limitations of phage and phage-encoded protein applications. Curr Protein Pept Sci. Bacteriophages and phage-derived proteins—application approaches.

HMMER web server: interactive sequence similarity searching. Cell lysis by induction of cloned lambda lysis genes. Mol Gen Genet.